Offered Programmes

Certificate course in Plant Tissue Culture and Micropropagation

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Programme Overview

Plant Tissue culture is an important tool for both basic and applied aspects of plant biotechnology as well as its commercial applications. As a technique widely known for the production of large numbers of genetically identical plantlets, this technology exhibits several advantages over conventional propagation techniques. All techniques are skill based and upon systematic learning, can equip a person to effectively utilize the techniques in various areas like basic research, environmental issues and commercial applications. It is a valuable tool for research on morphogenesis, cell signaling, physiology, and molecular biology, as well as crop improvement by biotechnology. This course offers a comprehensive hands-on training for learning the basics with an insight to laboratory practices along with exposure to “lab-to-land” transfers.


The objective of the course is to provide basic and applied training in the subject for development of skills for a successful career in entrepreneurship, generate technically trained human resource for tissue culture industries and as instructors in schools and junior colleges. Benefits of Course: The course will be a value added course for High School and Jr. College Science teachers. Entrepreneurs who wish to establish their own labs will be benefitted with the lab to land training; Researchers in plant tissue culture who have a focus on commercial applications such as crop improvement, secondary metabolite production, and various strategies for inducing genetic interference; persons who want to understand basic laboratory setup, handling of explant tissue, nutrient medium and establishing the culture, and incubation of cultures.

Course Structure

S.No. Course Title Total number of hours
1. PTC-1.1 Plant Tissue Culture: Techniques (T) 22 hrs
2. PTC-1.2 Micro-propagation (T) 22 hrs
3. PTC-2.1 Practical course based on PTC-1.1 80 hrs
4. PTC-2.2 Practical course based on PTC-1.2 80 hrs

Course Fees:


Course content

PTC-1.1:Plant Tissue Culture
  • iconIntroduction. History. Scope. Advantages. Applications. Limitations.
  • iconGuidelines for establishing academic and commercial laboratory.
  • iconSteps involved in Plant Tissue Culture.
  • iconVarious nutrient medium composition.
  • iconPlant growth regulators and their role in nutrient media.
  • iconTypes of organ cultures and their applications.
  • iconPathways of regeneration.
  • iconMicrografting of commercially important plants. In vitro approaches for crop improvement.
  • iconPlant cell reactors.
  • iconMicrotechniques
PTC-1.2:Micropropagation (22L)
  • iconMeristem culture for the production of virus free plants.
  • iconNucellus culture for clonal propagation and large scale multiplication.
  • iconStratagies of Micropropagation.
  • iconStages of micropropagation via axillary shoot proliferation in Monocots and Dicots.
  • iconStages in micropropagation via direct and indirect organogenesis.
  • iconStages in micropropagation via direct and indirect somatic embryogenesis.
  • iconLow cost methods for micropropagation.
PTC-2.1:Practicals in Pant Tissue Culture:
  • iconHandling and Instrumentation of Plant Tissue Culture.
  • iconGlassware Washing & Sterilization Techniques.
  • iconPreparation of stock solutions and nutrient media.
  • iconSurface sterilization of Explants.
  • iconMonocot and Dicot Seed cultures for the establishment of organ cultures.
  • iconEstablishment of organ cultures for the induction of callus
  • iconEstablishment of organ cultures for the induction of multiple shoots
  • iconSub-culturing of nucellar seedlings for clonal propagation.
  • iconTechnique of micrograftingin fruit crops.
  • iconPrimary hardeningof tissue culture plants for their acclimatization
  • iconCytological study of calli cells and their subculturing
  • iconEstablishment of embryogenic/ non embryogenic cell suspension cultures.
  • iconEmbryo / Endosperm/ Ovules and anther Cultures.
PTC-2.2: Practicals in Micropropagation
  • iconDemonstration of meristematic explants in different plants
  • iconPreparation of mother plants for collection of explants
  • iconMonocot shoot bud culture for axillary shoot proliferation
  • iconDicot Shoot tip / nodal segment culture for axillary shoot proliferation.
  • iconEstablishment of Dicot and Monocot multicultures
  • iconIn vitro and ex vitro rooting ofmicroshoots.
  • iconPrimary and secondary hardening of tissue cultured plants.
  • iconArtificial seed production from microshootsand theirin vitro germination.
  • iconEstablishment of cultures for the induction of embryogenic callus.
  • iconMaturation and conversion of somatic embryos into plantlets.
Admission Enquiry Apply Now

Key Information


6 Months


Min 12th Pass, preferably with Science subjects

course detail

Career Prospects

After completing their Advanced Diploma in Bioinformatics course, students can either opt for higher education like M.Phil or Ph.D in Bioinformatics or they can look for different career opportunities available after completing Diploma in Bioinformatics. Some career prospects after Advanced Diploma in Bioinformatics have been listed below. 

  • Consultancy / Private Practice
  • Employment in a Medical college, hospital, research institute, healthcare organisations etc.